miR-183 as a prognostic marker in intervertebral disc degeneration and its role in the proliferation and apoptosis of nucleus pulposus
Background Accumulating evidence indicated that microRNAs are involved in the pathogenesis of intervertebral disc degeneration (IDD). The roles of miR-183 in IDD have not been elucidated. Methods A total number of 60 patients with IDD were included in this study, and degenerative nucleus pulposus (NP) tissues of the patients were collected. Total RNA was extracted from NP of patients, and the expression levels of miR-183 were examined using real-time quantitative PCR methods. Next, human NP cells were cultured, and transfected with miR-183 mimics and inhibitors, and the roles of miR-183 on the proliferation and apoptosis of cells were determined using MTT and flow cytometry methods; moreover, the expression of MMP9, Caspase-3, BAX, and BCL-2 were examined using RT-qPCR and western blot methods. Results Patients with lower miR-183 level has better prognosis compared with patients with higher miR-183 level; moreover, transfection of miR-183 inhibitors induced significant increase in the proliferation and marked decrease in the apoptosis of the degenerated human NP cells; moreover, transfection of miR-183 inhibitors induced significant decrease in the expression of MMP9, Caspase-3, BAX, and marked increase in the expression of BCL-2 on both mRNA and protein level. Conclusion miR-183 was up-regulated in IDD, and was negatively associated with the prognosis of patients; moreover, miR-183 might regulate the proliferation and apoptosis of NP cells.
Keywords: miR-183, prognosis, IDD, proliferation, apoptosis