Roles of miR-128 in the pathogenesis of constipation and the related mechanism

Author(s): Cai He, Tao Zhou*, Li Liao

Abstract

The aim of this study is to explore the relationship between miR-128 and interstitial cells of Cajal (ICCs) in the pathogenesis of constipation and the related mechanism. We observed that the expression of miR-128 and c-kit were significantly down-regulated in constipated rats compared with the control rats; moreover, transient knockdown of miR-128 induced significant increase in the apoptosis and marked decrease in the proliferation of ICCs; finally, transfection of miR-128 inhibitors also lead to increased expression of Bax and Caspase-3, as well as reduced expression of Bcl-2 in ICCs. Taken together, our results proved for the first time that miR-128 can regulate the proliferation and apoptosis of ICCs via Bcl-2/Bax/Caspase-3 signaling pathway, suggesting that miR-128 has the potential to become a novel therapeutic target for the treatment of constipation.


Keywords: miR-128, ICCs, constipation, proliferation, apoptosis
Adv.Bio.Med.: 2019, Vol.4(No.1),pp:19-30
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