The neuroprotective effects of Quercetin on hypoxic-ischemic brain injury in neonatal mice

Author(s): Yiwen Zhang*, Yan Xiong, Hongbo Pang

Abstract:

Aim: To investigate the effects of quercetin on hypoxic-ischemic injury (HI) in neonatal mice and the mechanisms of action. Methods: 40 male C57BL/6J neonatal mice aging seven days were randomly divided into 4 groups: a sham with normal saline group (SOS), a sham with Quercetin group (SOQ), a hypoxia/ischemia with normal saline group (IHS), and a hypoxia/ischemia with quercetin group (IHQ). Then, 72 h after HI, mice were sacrificed to collect the brain tissues. Measuring the IL-1α and IL-6 concentrations by Elisa assay in brain. The nerve cell apoptosis of mice brain were measured by TUNEL assay. The IKK-β and NF-κB proteins expressions were measured by Immunohistochemistry (IHC) assay. Area of infarction and cerebral damage were measured by Nissl staining. Results: HIE induced neuronal death and tissue necrosis. Compared with IHS group, neuronal death and infarction area were significantly reduced in the IHQ group (P<0.05, respectively). Compared with IHS group, the IL-1α and IL-6 were significantly depressed, and IKK-β/NF-κB pathway were significantly suppressed in IHQ group (P<0.05, respectively). Conclusion: Quercetin improved HIE induced brain injury via IKK-β/NF-κB pathway.

Key words: Quercetin, HIE, IKK-β, NF-κB
Adv.Bio.Med.: 2018, Vol.3(No.4),pp:26-41
DOI: