Knockdown of MicroRNA-92a suppresses arcinogenic cellular activities of gastric cancer cells via targeting Bim

Author(s): Hao Huang*, Yang Chen, Zhonghua Chen

Abstract Aim: This study aimed to explain the effects and mechanisms of microRNA-92a in gastric cancer. Methods: Thirty pairs of cancerous and adjacent normal tissues were collected from patients with gastric cancer. BCL2-interacting mediator of cell death (Bim) and miRNA-92a expressions were evaluated using immunohistochemistry and in situ hybridisation, respectively. SGC7901 cells were divided into three groups: control, miRNA inhibitor, and miRNA inhibitor + BIM inhibitor. Cell proliferation and apoptosis were measured using MTT assay and flow cytometry, respectively. Cell invasiveness and migration were evaluated using transwell and wound-healing assays, respectively. Relative BIM, BCL-2 and EGFR expressions were assessed using western blotting. Results: Bim and miRNA-92a expressions in tumor tissues significantly up-regulated compared with adjacent normal tissues (P0.05, respectively). Compared with control group, cell proliferation and apoptosis in miRNA inhibitor group was significantly suppressed and elevated (P0.01, respectively); Cell invasiveness and migration in the miRNA inhibitor group were significantly suppressed compared with those in the control group (P0.01, respectively). BCL-2 and EGFR expressions in the miRNA inhibitor group was significantly suppressed compared with those in the control group, whereas BIM expression was significantly elevated in the miRNA inhibitor group compared with that in the control group (P 0.01). Conclusion: miRNA-92a knock-down thus suppresses in vitro SGC7901 cell activities in gastric cancer via BIM targeting.

Key words: miRNA-92a, Bim, Bcl-2, EGFR
Adv.Bio.Med.: 2018, Vol.3(No.3),pp:56-71