miR-206 inhibits high glucose-induced cell proliferation and extracellular matrix accumulation in mice glomerular mesangial cells
Abstract: The aim of this study was to investigate the effects of miR-206 on the proliferation and extracellular matrix (ECM) excessive accumulation of diabetic renal mesangial cells in mice with diabetic nephropathy. Mesangial cells (MCs) were cultured with high glucose to mimic diabetes mellitus. MCs were then transfected with miR-206 mimics. MTT and flow cytometry were used to detect the effects of miR-206 on the proliferation and cell cycle of mesangial cells. The impact of miR-206 level on expression of ERK signal pathway proteins and extracellular matrix proteins such as collagen IV, fibronectin were detected by real-time PCR and western blot. The results showed that high glucose could activate ERK signal transduction pathway in MCs, resulting in increased the expression levels of collagen IV, fibronectin. However, the overexpression of miR-206 obviously inhibited the proliferation of ECM cells, significantly decreased the phosphorylation level of ERK signaling pathway (P<0.05), and effectively decreased the secretion and expression of ECM protein (P<0.05). In conclusion, miR-206 may be a potential target for the prevention and therapy of diabetic nephropathy by inhibiting the accumulation of ECM via ERK signaling pathway activated by high glucose condition.
Keyword: miR-206, Diabetic nephropathy, Mesangial cells, Extracellular matrix, ERK