miR-96 affects the proliferation and metastasis of colon cancer by targeting forkhead box F2
Abstract: Numerous studies have reported that miRNA-96 are involved in the development and progression of various types of human cancers. In the current study, the roles of miR-96 in colon cancer has been investigated. Human colon cancer cell line SW480 cells were transfected with miR-96 inhibitors, and the effect of miR-96 on the proliferation, apoptosis, migration and invasion were examined. Moreover, the targeting relationship between miR-96 and FOXF2 was examined via luciferase reporter assay. Finally, RT-PCR and western blot methods have been applied to detect the effect of miR-96 on the expression of FOXF2 and Wnt/β-catenin signalling. We found that inhibition of the expression of miR-96 could inhibit the proliferation, invasion and migration, and promote the apoptosis of colon cancer cell line SW480 cells. We verified that FOXF2 was the direct target of miR-96 through transfecting miR-96 mimics and luciferase reporter assay. qRT-PCR analysis and Western Blot revealed that inhibition of the expression of miR-96 could affect the expression of FOXF2, GSK-3β and β-catenin on mRNA and protein level. In conclusion, we reported for the first time that down-regulation of miR-96 could inhibit cell proliferation, migration, and invasion of colon cancer through targeting FOXF2 by inhibiting β-catenin signaling pathway.
Key word: miR-96, colon cancer, Wnt/β-catenin, FOXF2