MicroRNA-375 regulates proliferation and apoptosis of colon cancer by suppressing CTGF-EGFR signaling pathway
Abstract: Aim: The aim of this study was to explain the effect and mechanism of microRNA-375 in colon cancer. Methods: The HT29 and SW620 cells were respectively divided into 3 groups: NC group, BL group and miRNA group. We measured the cell proliferation, apoptosis and cell cycle of difference groups. The relative proteins expressions were evaluated by WB assay. Results: Compared with NC groups, the cell proliferation of miRNA groups were significantly suppressed (P＜0.05, respectively), the cell apoptosis of miRNA groups were significantly enhanced (P＜0.05, respectively) and the G1 phased rate of miRNA groups were significantly increased (P＜0.05, respectively) in HT29 and SW620 cells. To explain the mechanism of miRNA-375 in colon cancer, the relative proteins expressions were measured by WB assay. The CTGF, EGFR, PI3K, AKT and ERK proteins expressions of miRNA-375 groups were significantly suppressed compared with NC groups (P＜0.05, respectively). Conclusion: miRNA-375 overexpression had anti-tumor effects to colon cancer via CTGF-EGFR signaling pathway.
Key words: miRNA-375, CTGF, EGFR, PI3K, colon cancer